SEPARATION THE ENANTIOMERS OF DIFFERENT ANTIMYCOTIC DRUGS IN CAPILLARY ELECTROPHORESIS USING CARBOXYMETHYLATED β- CYCLODEXTRIN AS CHIRAL SELECTORS

Enantioseparations using capillary electrophoresis (CE) are actively studied nowadays not only for its highly efficient separation ability, but also for understanding non-covalent intermolecular interactions. As neither electrophoretic, nor electroosmotic mobility alone is enantioselective migration for enantiomers, addition of chiral selector is necessary for enantioseparations. [1] For this reason, native and substituted cyclodextrins are used, which bind selectively with one of the enantiomers. [2] Many diazole and triazole derivatives are widely used for pharmaceutical use as antimycotics. Some of the pharmaceutically used triazole derivatives contain a chiral carbon atom and therefore exist as racemic mixtures of the enantiomers. The enantiomers have different pharmacokinetic, pharmacodynamic and toxic properties. The purpose of our study was to find optimal conditions for separation of a 1,2,4-triazole based fungicide Penconazole enantiomers. It was found that baseline separation is obtained using carboxymethylated-β-cyclodextrin at pH of 2.12. However, migration time is very long. For this reason, experiments were performed on higher pH values, as the given cyclodextrin is deprotonated and the mobility of the selector-enantiomer complex can be increased.